Volet 1 TP 2

Évaluation de l’introgression potentielle à partir de plantations d’hybrides de peuplier à composantes exotiques.

Responsable(s) : Nathalie Isabel, Damase Khasa, Jean Bousquet

Collaborateur(s) : Pierre Périnet, Patrick Meirmans, Gabriela Guigou, Marie-Claude Gros-Louis

Objectifs :

1. développer et optimiser des marqueurs moléculaires spécifiques aux peupliers;
2. évaluer la contamination (pollution) du pool génique de peupliers indigènes par les peupliers à composantes exotiques;
3. intégrer les données génétiques et écologiques dans un modèle de simulation spatiale.

Publications produites dans le cadre du TP :

• Damase Khasa, Patrick Pollefeys, Aura Navarro-Quezada, Pierre Périnet, Jean Bousquet. 2005. Species-specific microsatellite markers to monitor gene flow between exotic poplars and their natural relatives in eastern North America. Molecular Ecology Notes 5(4):920-923 doi:10.1111/j.1471-8286.2005.01114.x. Cette publication est réalisée dans le cadre du Volet 1 TP 2 (résumé)

  Species-specific microsatellite markers were obtained for the unambiguous recognition of five poplar species of ecological and commercial importance to eastern North America: the native species Populus balsamifera and Populus deltoides, the exotic species Populus maximowiczii, Populus nigra, Populus trichocarpa and their interspecific hybrids. Forty-four of 71 tested primer pairs amplified simple sequence repeat (SSR) loci for all five taxa. Six of these loci showed non-overlapping allelic diversity between species, including fixed differences. Together, they were useful to identify unambiguously the five taxa and to validate parental contributions in a group of hybrid progeny. These markers will be invaluable to detect gene flow from plantations of exotic poplar into adjacent stands of native species and between the two potentially hybridizing native species P. balsamifera and P. deltoides.